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An Algorithm Oriented at Obtaining the Molecular Weight and Concentration of DNA Samples in Agarose Gel Images

  • Universidad Peruana de Ciencias Aplicadas
  • Universidad Nacional de Ingeniería, Peru

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This article presents an algorithm for obtaining the weight and concentration of DNA samples from agarose gel images using digital image processing techniques. The purpose of this algorithm is to obtain much more accurate results than the results obtained by experts using their visual sense. The algorithm is based on capturing the DNA samples and comparing them with DNA molecular weight markers using digital image processing techniques to find the concentration, which is the intensity of the samples, and the weight of the samples. The proposed algorithm achieved a moderate “level of agreement” in the results, as calculated by kappa, compared with the expert results of a total of six images, containing 51 DNA samples in total. These DNA samples open source is from flies.

Original languageEnglish
Title of host publicationProceedings of the 5th Brazilian Technology Symposium - Emerging Trends, Issues, and Challenges in the Brazilian Technology
EditorsYuzo Iano, Rangel Arthur, Osamu Saotome, Guillermo Kemper, Ana Carolina Borges Monteiro
PublisherSpringer Science and Business Media Deutschland GmbH
Pages61-72
Number of pages12
ISBN (Print)9783030575656
DOIs
StatePublished - 2021
Event5th Brazilian Technology Symposium, BTSym 2019 - Campinas, Brazil
Duration: 22 Oct 201924 Oct 2019

Publication series

NameSmart Innovation, Systems and Technologies
Volume202
ISSN (Print)2190-3018
ISSN (Electronic)2190-3026

Conference

Conference5th Brazilian Technology Symposium, BTSym 2019
Country/TerritoryBrazil
CityCampinas
Period22/10/1924/10/19

Keywords

  • Agarose gel
  • DNA
  • DNA ladder
  • Image processing
  • Molecular weight

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