An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection

Carmen C. Mayorga-Martinez; Alejandro Chamorro-García; Lorena Serrano; Lourdes Rivas; Daniel Quesada-Gonzalez; Laura Altet; Olga Francino; Armand Sánchez; Arben Merkoçi

doi:10.1039/c5tb00545k

An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection

Carmen C. Mayorga-Martinez
, Alejandro Chamorro-García
, Lorena Serrano
, Lourdes Rivas
, Daniel Quesada-Gonzalez
, Laura Altet
, Olga Francino
, Armand Sánchez
, Arben Merkoçi

Catalan Institute of Nanoscience and Nanotechnology
Autonomous University of Barcelona
ICREA

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO₂ NP). An aminated oligonucleotide probe is immobilized on the IrO₂ NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD ≈ 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.

Original language	English
Pages (from-to)	5166-5171
Number of pages	6
Journal	Journal of Materials Chemistry B
Volume	3
Issue number	26
DOIs	https://doi.org/10.1039/c5tb00545k
State	Published - 14 Jul 2015
Externally published	Yes

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title = "An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection",

abstract = "An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO2 NP). An aminated oligonucleotide probe is immobilized on the IrO2 NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD ≈ 15\% and sensitive enough while using 2\% of ethanolamine during the blocking step employed for genosensor preparation.",

author = "Mayorga-Martinez, \{Carmen C.\} and Alejandro Chamorro-Garc{\'i}a and Lorena Serrano and Lourdes Rivas and Daniel Quesada-Gonzalez and Laura Altet and Olga Francino and Armand S{\'a}nchez and Arben Merko{\c c}i",

note = "Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",

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doi = "10.1039/c5tb00545k",

language = "Ingl{\'e}s",

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TY - JOUR

T1 - An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection

AU - Mayorga-Martinez, Carmen C.

AU - Chamorro-García, Alejandro

AU - Serrano, Lorena

AU - Rivas, Lourdes

AU - Quesada-Gonzalez, Daniel

AU - Altet, Laura

AU - Francino, Olga

AU - Sánchez, Armand

AU - Merkoçi, Arben

PY - 2015/7/14

Y1 - 2015/7/14

N2 - An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO2 NP). An aminated oligonucleotide probe is immobilized on the IrO2 NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD ≈ 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.

AB - An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO2 NP). An aminated oligonucleotide probe is immobilized on the IrO2 NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD ≈ 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.

UR - https://www.scopus.com/pages/publications/84933054646

U2 - 10.1039/c5tb00545k

DO - 10.1039/c5tb00545k

M3 - Artículo

AN - SCOPUS:84933054646

SN - 2050-7518

VL - 3

SP - 5166

EP - 5171

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

IS - 26

ER -

An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection

Abstract

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