ARTIFICIAL PROTEIN SENSOR FOR PROTEASE ACTIVITY AND INHIBITION

Katherin Lizet Peñaranda Manrique; Pohl Luis Milon Mayer; Cesar Gabriel Mendoza Rojas

ARTIFICIAL PROTEIN SENSOR FOR PROTEASE ACTIVITY AND INHIBITION

Katherin Lizet Peñaranda Manrique (Inventor)
, Pohl Luis Milon Mayer (Inventor)
, Cesar Gabriel Mendoza Rojas (Inventor)

Faculty of Health Sciences

UNIVERSIDAD PERUANA DE CIENCIAS APLICADAS S.A.C

Research output: Patent

Abstract

The present invention discloses a reliable protease activity biosensor for determining the cleavage of a protease recognition site that can be viewed by FRET, fluorescence change or by SDS-PAGE electrophoresis. This biosensor consists of a globular domain, a flexible connector with a variable protease recognition/cleavage area and a second globular domain. The proposed invention can be useful for detecting proteases and assessing the activity thereof, as well as finding novel protease inhibitors or monitoring the efficiency of therapeutic agents in patients undergoing treatment. The proposed biosensor is an engineered protein from the rational engineering of proteins which makes this molecule unique and not available in nature.

Original language	American English
Patent number	WO2025089973
IPC	C12Q 1/ 37 A I
Priority date	24/10/23
State	Published - 1 May 2025

Cite this

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title = "ARTIFICIAL PROTEIN SENSOR FOR PROTEASE ACTIVITY AND INHIBITION",

abstract = "The present invention discloses a reliable protease activity biosensor for determining the cleavage of a protease recognition site that can be viewed by FRET, fluorescence change or by SDS-PAGE electrophoresis. This biosensor consists of a globular domain, a flexible connector with a variable protease recognition/cleavage area and a second globular domain. The proposed invention can be useful for detecting proteases and assessing the activity thereof, as well as finding novel protease inhibitors or monitoring the efficiency of therapeutic agents in patients undergoing treatment. The proposed biosensor is an engineered protein from the rational engineering of proteins which makes this molecule unique and not available in nature.",

author = "\{Pe{\~n}aranda Manrique\}, \{Katherin Lizet\} and \{Milon Mayer\}, \{Pohl Luis\} and \{Mendoza Rojas\}, \{Cesar Gabriel\}",

year = "2025",

month = may,

day = "1",

language = "American English",

type = "Patent",

note = "WO2025089973; C12Q 1/ 37 A I",

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TY - PAT

T1 - ARTIFICIAL PROTEIN SENSOR FOR PROTEASE ACTIVITY AND INHIBITION

AU - Peñaranda Manrique, Katherin Lizet

AU - Milon Mayer, Pohl Luis

AU - Mendoza Rojas, Cesar Gabriel

PY - 2025/5/1

Y1 - 2025/5/1

N2 - The present invention discloses a reliable protease activity biosensor for determining the cleavage of a protease recognition site that can be viewed by FRET, fluorescence change or by SDS-PAGE electrophoresis. This biosensor consists of a globular domain, a flexible connector with a variable protease recognition/cleavage area and a second globular domain. The proposed invention can be useful for detecting proteases and assessing the activity thereof, as well as finding novel protease inhibitors or monitoring the efficiency of therapeutic agents in patients undergoing treatment. The proposed biosensor is an engineered protein from the rational engineering of proteins which makes this molecule unique and not available in nature.

AB - The present invention discloses a reliable protease activity biosensor for determining the cleavage of a protease recognition site that can be viewed by FRET, fluorescence change or by SDS-PAGE electrophoresis. This biosensor consists of a globular domain, a flexible connector with a variable protease recognition/cleavage area and a second globular domain. The proposed invention can be useful for detecting proteases and assessing the activity thereof, as well as finding novel protease inhibitors or monitoring the efficiency of therapeutic agents in patients undergoing treatment. The proposed biosensor is an engineered protein from the rational engineering of proteins which makes this molecule unique and not available in nature.

M3 - Patent

M1 - WO2025089973

ER -

ARTIFICIAL PROTEIN SENSOR FOR PROTEASE ACTIVITY AND INHIBITION

Abstract

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