Caracterización microbiológica y molecular de la resistencia antimicrobiana de escherichia coli uropatógenas de hospitales públicos peruanos

Pool Marcos-Carbajal; Guillermo Salvatierra; José Yareta; Jimena Pino; Nancy Vásquez; Pilar Diaz; Isabel Martínez; Percy Asmat; Carlos Peralta; Caridad Huamani; Alexander Briones; Manuel Ruiz; Nicomedes Laura; Álvaro Luque; Leonel Arapa; Pablo Tsukayama

doi:10.17843/rpmesp.2021.381.6182

Caracterización microbiológica y molecular de la resistencia antimicrobiana de escherichia coli uropatógenas de hospitales públicos peruanos

Translated title of the contribution: Microbiological and molecular characterization of antimicrobial resistance in uropathogenic escherichia coli from peruvian public hospitals

Pool Marcos-Carbajal
, Guillermo Salvatierra
, José Yareta
, Jimena Pino
, Nancy Vásquez
, Pilar Diaz
, Isabel Martínez
, Percy Asmat
, Carlos Peralta
, Caridad Huamani
, Alexander Briones
, Manuel Ruiz
, Nicomedes Laura
, Álvaro Luque
, Leonel Arapa
, Pablo Tsukayama

Universidad Peruana Unión
Universidad Peruana Cayetano Heredia
Ministerio de Salud del Perú
Laboratorio de Referencia Regional Salud Pública San Martín
Laboratorio de Referencia Regional de Salud Pública Tumbes
Laboratorio de Referencia Regional Salud Pública
IPRESS Jorge Chávez
Hospital Regional de Loreto
Área de Microbiología
Laboratorio de Referencia Regional de Salud Pública Huancavelica
Clínica Americana Juliaca
Hospital Carlos Monge Medrano

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

We characterized the antimicrobial resistance of 70 Escherichia coli isolates obtained from patients with a urinary tract infection (UTI) from 8 public hospitals in Peru. Resistance profiles were identified using the automated MicroScan® system. A standard polymerase chain reaction was used for the detection of the bla_TEM, bla_CTX-M, bla_SHV and bla_PER genes. The 65.7% (46/70) of the isolates presented a multidrug-re-sistant phenotype and 55.7% (39/70) were extended-spectrum beta-lactamases producers. High levels of resistance were detected for ampicillin (77,1%), ciprofloxacin (74,3%), trimethoprim/sulfamethoxazole (62,9%), cefepime (57,1%), and cefuroxime (57,1%). The bla_TEM gene was the most frequent (31,4%), followed by bla_CTX-M (18,6%) and bla_SHV (2,9%) genes. These results show high resistance levels to antimi-crobials of clinical use in E. coli isolates from hospital UTI patients in Peru.

Translated title of the contribution	Microbiological and molecular characterization of antimicrobial resistance in uropathogenic escherichia coli from peruvian public hospitals
Original language	Spanish
Pages (from-to)	119-123
Number of pages	5
Journal	Revista Peruana de Medicina Experimental y Salud Publica
Volume	38
Issue number	1
DOIs	https://doi.org/10.17843/rpmesp.2021.381.6182
State	Published - 2021
Externally published	Yes

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SDG 3 Good Health and Well-being

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10.17843/rpmesp.2021.381.6182

Cite this

Marcos-Carbajal, P., Salvatierra, G., Yareta, J., Pino, J., Vásquez, N., Diaz, P., Martínez, I., Asmat, P., Peralta, C., Huamani, C., Briones, A., Ruiz, M., Laura, N., Luque, Á., Arapa, L., & Tsukayama, P. (2021). Caracterización microbiológica y molecular de la resistencia antimicrobiana de escherichia coli uropatógenas de hospitales públicos peruanos. Revista Peruana de Medicina Experimental y Salud Publica, 38(1), 119-123. https://doi.org/10.17843/rpmesp.2021.381.6182

@article{e96099cfc88a4b4089f6601072e82dff,

title = "Caracterizaci{\'o}n microbiol{\'o}gica y molecular de la resistencia antimicrobiana de escherichia coli uropat{\'o}genas de hospitales p{\'u}blicos peruanos",

abstract = "We characterized the antimicrobial resistance of 70 Escherichia coli isolates obtained from patients with a urinary tract infection (UTI) from 8 public hospitals in Peru. Resistance profiles were identified using the automated MicroScan{\textregistered} system. A standard polymerase chain reaction was used for the detection of the blaTEM, blaCTX-M, blaSHV and blaPER genes. The 65.7\% (46/70) of the isolates presented a multidrug-re-sistant phenotype and 55.7\% (39/70) were extended-spectrum beta-lactamases producers. High levels of resistance were detected for ampicillin (77,1\%), ciprofloxacin (74,3\%), trimethoprim/sulfamethoxazole (62,9\%), cefepime (57,1\%), and cefuroxime (57,1\%). The blaTEM gene was the most frequent (31,4\%), followed by blaCTX-M (18,6\%) and blaSHV (2,9\%) genes. These results show high resistance levels to antimi-crobials of clinical use in E. coli isolates from hospital UTI patients in Peru.",

keywords = "Beta-Lactam Resistance, Drug Resistance, Escherichia coli, Peru, Urologic Diseases",

author = "Pool Marcos-Carbajal and Guillermo Salvatierra and Jos{\'e} Yareta and Jimena Pino and Nancy V{\'a}squez and Pilar Diaz and Isabel Mart{\'i}nez and Percy Asmat and Carlos Peralta and Caridad Huamani and Alexander Briones and Manuel Ruiz and Nicomedes Laura and {\'A}lvaro Luque and Leonel Arapa and Pablo Tsukayama",

year = "2021",

doi = "10.17843/rpmesp.2021.381.6182",

language = "Espa{\~n}ol",

volume = "38",

pages = "119--123",

journal = "Revista Peruana de Medicina Experimental y Salud Publica",

issn = "1726-4634",

publisher = "Instituto Nacional de Salud",

number = "1",

}

Marcos-Carbajal, P, Salvatierra, G, Yareta, J, Pino, J, Vásquez, N, Diaz, P, Martínez, I, Asmat, P, Peralta, C, Huamani, C, Briones, A, Ruiz, M, Laura, N, Luque, Á, Arapa, L & Tsukayama, P 2021, 'Caracterización microbiológica y molecular de la resistencia antimicrobiana de escherichia coli uropatógenas de hospitales públicos peruanos', Revista Peruana de Medicina Experimental y Salud Publica, vol. 38, no. 1, pp. 119-123. https://doi.org/10.17843/rpmesp.2021.381.6182

Caracterización microbiológica y molecular de la resistencia antimicrobiana de escherichia coli uropatógenas de hospitales públicos peruanos. / Marcos-Carbajal, Pool; Salvatierra, Guillermo; Yareta, José et al.
In: Revista Peruana de Medicina Experimental y Salud Publica, Vol. 38, No. 1, 2021, p. 119-123.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Caracterización microbiológica y molecular de la resistencia antimicrobiana de escherichia coli uropatógenas de hospitales públicos peruanos

AU - Marcos-Carbajal, Pool

AU - Salvatierra, Guillermo

AU - Yareta, José

AU - Pino, Jimena

AU - Vásquez, Nancy

AU - Diaz, Pilar

AU - Martínez, Isabel

AU - Asmat, Percy

AU - Peralta, Carlos

AU - Huamani, Caridad

AU - Briones, Alexander

AU - Ruiz, Manuel

AU - Laura, Nicomedes

AU - Luque, Álvaro

AU - Arapa, Leonel

AU - Tsukayama, Pablo

PY - 2021

Y1 - 2021

N2 - We characterized the antimicrobial resistance of 70 Escherichia coli isolates obtained from patients with a urinary tract infection (UTI) from 8 public hospitals in Peru. Resistance profiles were identified using the automated MicroScan® system. A standard polymerase chain reaction was used for the detection of the blaTEM, blaCTX-M, blaSHV and blaPER genes. The 65.7% (46/70) of the isolates presented a multidrug-re-sistant phenotype and 55.7% (39/70) were extended-spectrum beta-lactamases producers. High levels of resistance were detected for ampicillin (77,1%), ciprofloxacin (74,3%), trimethoprim/sulfamethoxazole (62,9%), cefepime (57,1%), and cefuroxime (57,1%). The blaTEM gene was the most frequent (31,4%), followed by blaCTX-M (18,6%) and blaSHV (2,9%) genes. These results show high resistance levels to antimi-crobials of clinical use in E. coli isolates from hospital UTI patients in Peru.

AB - We characterized the antimicrobial resistance of 70 Escherichia coli isolates obtained from patients with a urinary tract infection (UTI) from 8 public hospitals in Peru. Resistance profiles were identified using the automated MicroScan® system. A standard polymerase chain reaction was used for the detection of the blaTEM, blaCTX-M, blaSHV and blaPER genes. The 65.7% (46/70) of the isolates presented a multidrug-re-sistant phenotype and 55.7% (39/70) were extended-spectrum beta-lactamases producers. High levels of resistance were detected for ampicillin (77,1%), ciprofloxacin (74,3%), trimethoprim/sulfamethoxazole (62,9%), cefepime (57,1%), and cefuroxime (57,1%). The blaTEM gene was the most frequent (31,4%), followed by blaCTX-M (18,6%) and blaSHV (2,9%) genes. These results show high resistance levels to antimi-crobials of clinical use in E. coli isolates from hospital UTI patients in Peru.

KW - Beta-Lactam Resistance

KW - Drug Resistance

KW - Escherichia coli

KW - Peru

KW - Urologic Diseases

UR - https://www.scopus.com/pages/publications/85111789116

U2 - 10.17843/rpmesp.2021.381.6182

DO - 10.17843/rpmesp.2021.381.6182

M3 - Artículo

C2 - 34190903

AN - SCOPUS:85111789116

SN - 1726-4634

VL - 38

SP - 119

EP - 123

JO - Revista Peruana de Medicina Experimental y Salud Publica

JF - Revista Peruana de Medicina Experimental y Salud Publica

IS - 1

ER -

Caracterización microbiológica y molecular de la resistencia antimicrobiana de escherichia coli uropatógenas de hospitales públicos peruanos

Abstract

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