UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection

Roberto Alcántara; Katherin Peñaranda; Gabriel Mendoza-Rojas; Jose A. Nakamoto; Eva Dueñas; Daniela Alvarez; Vanessa Adaui; Pohl Milón

doi:10.1016/j.xpro.2021.100878

UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection

Roberto Alcántara
, Katherin Peñaranda
, Gabriel Mendoza-Rojas
, Jose A. Nakamoto
, Eva Dueñas
, Daniela Alvarez
, Vanessa Adaui
, Pohl Milón

Universidad Peruana de Ciencias Aplicadas

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternatively, a fluorescence plate reader can be used for quantitative measurements. The protocol detects two regions of the SARS-CoV-2 genome in addition to the human RNaseP sample control. Aiming to reach remote regions, this work was developed to use the portable molecular workstation from BentoLab. For complete details on the use and execution of this protocol, please refer to Alcántara et al., 2021.

Original language	English
Article number	100878
Journal	STAR Protocols
Volume	2
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2021.100878
State	Published - 17 Dec 2021

Keywords

Biotechnology and bioengineering
CRISPR
Clinical Protocol
Immunology
Microbiology
Molecular Biology

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10.1016/j.xpro.2021.100878

Cite this

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title = "UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection",

abstract = "Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternatively, a fluorescence plate reader can be used for quantitative measurements. The protocol detects two regions of the SARS-CoV-2 genome in addition to the human RNaseP sample control. Aiming to reach remote regions, this work was developed to use the portable molecular workstation from BentoLab. For complete details on the use and execution of this protocol, please refer to Alc{\'a}ntara et al., 2021.",

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T2 - A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection

AU - Alcántara, Roberto

AU - Peñaranda, Katherin

AU - Mendoza-Rojas, Gabriel

AU - Nakamoto, Jose A.

AU - Dueñas, Eva

AU - Alvarez, Daniela

AU - Adaui, Vanessa

AU - Milón, Pohl

PY - 2021/12/17

Y1 - 2021/12/17

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AB - Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternatively, a fluorescence plate reader can be used for quantitative measurements. The protocol detects two regions of the SARS-CoV-2 genome in addition to the human RNaseP sample control. Aiming to reach remote regions, this work was developed to use the portable molecular workstation from BentoLab. For complete details on the use and execution of this protocol, please refer to Alcántara et al., 2021.

KW - Biotechnology and bioengineering

KW - CRISPR

KW - Clinical Protocol

KW - Immunology

KW - Microbiology

KW - Molecular Biology

UR - https://www.scopus.com/pages/publications/85118485880

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DO - 10.1016/j.xpro.2021.100878

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AN - SCOPUS:85118485880

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M1 - 100878

ER -

UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection

Abstract

Keywords

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