An Algorithm Oriented at Obtaining the Molecular Weight and Concentration of DNA Samples in Agarose Gel Images

Christian del Carpio, Marcelo Garcia, Julio Elías, David Laván, Guillermo Kemper

Producción científica: Capítulo del libro/informe/acta de congresoContribución a la conferenciarevisión exhaustiva

Resumen

This article presents an algorithm for obtaining the weight and concentration of DNA samples from agarose gel images using digital image processing techniques. The purpose of this algorithm is to obtain much more accurate results than the results obtained by experts using their visual sense. The algorithm is based on capturing the DNA samples and comparing them with DNA molecular weight markers using digital image processing techniques to find the concentration, which is the intensity of the samples, and the weight of the samples. The proposed algorithm achieved a moderate “level of agreement” in the results, as calculated by kappa, compared with the expert results of a total of six images, containing 51 DNA samples in total. These DNA samples open source is from flies.

Idioma originalInglés
Título de la publicación alojadaProceedings of the 5th Brazilian Technology Symposium - Emerging Trends, Issues, and Challenges in the Brazilian Technology
EditoresYuzo Iano, Rangel Arthur, Osamu Saotome, Guillermo Kemper, Ana Carolina Borges Monteiro
EditorialSpringer Science and Business Media Deutschland GmbH
Páginas61-72
Número de páginas12
ISBN (versión impresa)9783030575656
DOI
EstadoPublicada - 2021
Evento5th Brazilian Technology Symposium, BTSym 2019 - Campinas, Brasil
Duración: 22 oct. 201924 oct. 2019

Serie de la publicación

NombreSmart Innovation, Systems and Technologies
Volumen202
ISSN (versión impresa)2190-3018
ISSN (versión digital)2190-3026

Conferencia

Conferencia5th Brazilian Technology Symposium, BTSym 2019
País/TerritorioBrasil
CiudadCampinas
Período22/10/1924/10/19

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