TY - JOUR
T1 - Multilocus genotyping reveals a polyphyletic pattern among naturally antimony-resistant Leishmania braziliensis isolates from Peru
AU - Adaui, Vanessa
AU - Maes, Ilse
AU - Huyse, Tine
AU - Van den Broeck, Frederik
AU - Talledo, Michael
AU - Kuhls, Katrin
AU - De Doncker, Simonne
AU - Maes, Louis
AU - Llanos-Cuentas, Alejandro
AU - Schönian, Gabriele
AU - Arevalo, Jorge
AU - Dujardin, Jean Claude
PY - 2011/12
Y1 - 2011/12
N2 - In order to understand the epidemiological dynamics of antimonial (Sb V) resistance in zoonotic tegumentary leishmaniasis and its link with treatment outcome, we analyzed the population structure of 24 Peruvian Leishmania braziliensis clinical isolates with known in vitro antimony susceptibility and clinical phenotype by multilocus microsatellite typing (14 microsatellite loci). The genetic variability in the Peruvian isolates was high and the multilocus genotypes were strongly differentiated from each other. No correlation was found between the genotypes and in vitro drug susceptibility or clinical treatment outcome. The finding of a polyphyletic pattern among the Sb V-resistant L. braziliensis might be explained by (i) independent events of drug resistance emergence, (ii) sexual recombination and/or (iii) other phenomena mimicking recombination signals. Interestingly, the polyphyletic pattern observed here is very similar to the one we observed in the anthroponotic Leishmania donovani (Laurent et al., 2007), hereby questioning the role of transmission and/or chemotherapeutic drug pressure in the observed population structure.
AB - In order to understand the epidemiological dynamics of antimonial (Sb V) resistance in zoonotic tegumentary leishmaniasis and its link with treatment outcome, we analyzed the population structure of 24 Peruvian Leishmania braziliensis clinical isolates with known in vitro antimony susceptibility and clinical phenotype by multilocus microsatellite typing (14 microsatellite loci). The genetic variability in the Peruvian isolates was high and the multilocus genotypes were strongly differentiated from each other. No correlation was found between the genotypes and in vitro drug susceptibility or clinical treatment outcome. The finding of a polyphyletic pattern among the Sb V-resistant L. braziliensis might be explained by (i) independent events of drug resistance emergence, (ii) sexual recombination and/or (iii) other phenomena mimicking recombination signals. Interestingly, the polyphyletic pattern observed here is very similar to the one we observed in the anthroponotic Leishmania donovani (Laurent et al., 2007), hereby questioning the role of transmission and/or chemotherapeutic drug pressure in the observed population structure.
KW - Antimony resistance
KW - Clinical isolates
KW - Leishmania braziliensis
KW - Microsatellite markers
KW - Population genetics
KW - Zoonotic tegumentary leishmaniasis
UR - https://www.scopus.com/pages/publications/82655172399
U2 - 10.1016/j.meegid.2011.08.008
DO - 10.1016/j.meegid.2011.08.008
M3 - Artículo
C2 - 21871584
AN - SCOPUS:82655172399
SN - 1567-1348
VL - 11
SP - 1873
EP - 1880
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
IS - 8
ER -
