@inbook{865872f7ed34498a98f7b2aa7bf63de3,
title = "Real-Time Dynamics of Ribosome-Ligand Interaction by Time-Resolved Chemical Probing Methods",
abstract = "Three protocols to perform time-resolved in situ probing of rRNA are described. The three methods (chemical modification with DMS and rRNA backbone cleavage by hydroxyl radicals generated by either K-peroxonitrite or Fe(II)-EDTA) make use of a quench-flow apparatus and exploit reactions that are faster than the interactions of ribosomal subunits with their ligands. These methods allow the investigation of the path and dynamics, in a ≅ 50 to 1500 ms time range, of the binding and dissociation of ribosomal ligands.",
author = "Attilio Fabbretti and Pohl Milon and Giuliodori, \{Anna Maria\} and Gualerzi, \{Claudio O.\} and Pon, \{Cynthia L.\}",
year = "2007",
doi = "10.1016/S0076-6879(07)30003-7",
language = "Ingl{\'e}s",
isbn = "9780123739698",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "45--58",
booktitle = "Translation Initiation",
}